Mouse anti‑Human Actin IgG2a,k Monoclonal RW-C389420-100

Antibody: Actin Mouse anti-Human Monoclonal (N-Terminus) (Azide-free, BSA-free) (1A4) Antibody, Application: IHC, IHC-P, IF, WB, IP, Flo, ELISA, Reactivity: Human, Baboon, Monkey, Mouse, Rat, Bovine, Cat, Dog, Goat, Guinea pig, Pig, Rabbit, Sheep, Chicken, Format: Unconjugated, Azide-free, BSA-free, Other formats: Ready-to-Use Unmodified, Descriptio: Actin antibody RW-C389420 is an unconjugated mouse monoclonal antibody to Actin (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IF, IHC, IP and WB., Targe: Human Actin, Hos: Mouse, Reactivit: Human, Baboon, Monkey, Mouse, Rat, Bovine, Cat, Dog, Goat, Guinea pig, Pig, Rabbit, Sheep, Chicken (tested or 100% immunogen sequence identity), Clonalit: IgG2a,k Monoclonal, Clon: 1A4, Conjugation: Unconjugated, Purificatio: Protein G purified, Modification: Azide-free, BSA-free. Also available Unmodified or Ready-to-Use., Immunoge: An N-Terminus decapeptide of the alpha smooth muscle isoform., Epitop: N-Terminus, Specificit: Human Actin, Application: IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Immunofluorescence (0.5 - 1 µg/ml)
Western blot
Immunoprecipitation
Flow Cytometry (0.5 - 1 µg/10E6 cells)
ELISA, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle. however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin. No special pretreatment is required for the immunohistochemical staining of FFPE tissue. ELISA: Order BSA-free format., Presentatio: 1X PBS, Storag: Store at 2°C to 8°C., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.